Catalogue number
PRO-2258
Synonyms
Tyrosine-protein
phosphatase non-receptor type substrate 1, SHP substrate, SHPS-1, Brain Ig-like
molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like
family member A, Inhibitory receptor
SHPS-1, Macrophage fusion receptor, Macrophage membrane protein MFP150, Signal-regulatory
protein alpha-1, Sirp-alpha-1, CD172a, Sirpa, Bit, Mfr, Ptpns1, Shps1, Sirp.
Introduction
Signal-Regulatory Protein Alpha, SIRPA belongs to the signal-regulatory-protein (SIRP) family, as well as theimmunoglobulin super family. The members of the SIRP family are receptor-type transmembrane glycoproteins which areinvolved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPA can bephosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2domain containing tyrosine phosphatases (PTP), and perform as substrates of PTPs. SIRPA take part insignal transduction mediated by a variety of growth factor receptors. CD47 has been shown to be a ligand forSIRPA.
Description
SIRPA Rat Recombinant produced in Sf9 Baculovirus cells
is a single, glycosylated polypeptide chain containing 350 amino acids (32-373a.a)
and having a molecular mass of 38.5kDa. (Migrates at 57-70kDa on SDS-PAGE under
reducing conditions). SIRPA is fused to an 8 amino acid His-tag at C-terminus
& purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
SIRPA protein solution (0.5mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Amino acid sequence
KELKVTQADK SVSVAAGDSA TLNCTVSSLT PVGPIKWFKG EGQNRSPIYS
FIGGEHFPRI TNVSDATKRN NMDFSICISN VTPEDAGTYY CVKFQKGIVE PDTEIKSGGG TTLYVLAKPS
SPEVSGPDSR GSPGQTVNFT CKSYGFSPRN ITLKWLKNGK ELSHLETTIS SKSNVSYNIS STVSVKLSPE DIHSRVICEV
AHVTLEGRPL NGTANFSNII RVSPTLKITQ QPLTPASQVN LTCQVQKFYP KALQLNWLEN GNLSRTDKPE HFTDNRDGTY
NYTSLFLVNS SAHREDVVFT CQVEHDSQPA ITENHTVRAF AHSSSGGSME TIPDNNAYYN WNVEHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
