Catalogue number
PRO-2131
Synonyms
Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa, RNA Polymerases I, II, And III Subunit ABC4, DNA-Directed RNA Polymerase II Subunit K, RNA Polymerase II 7.0 KDa Subunit, ABC10-Alpha, RPB10alpha, RPB7.0, RPB12,DNA Directed RNA Polymerases I, II, And III 7.0 Kda Polypeptide, DNA-Directed RNA Polymerases I, II, And III Subunit RPABC4, Polymerase (RNA) II (DNA Directed) Polypeptide K (7.0kD), HsRPB10a, HRPB7.0, RPABC4, POLR2K.
Introduction
Polymerase (RNA) II (DNA directed) Polypeptide K, also known as POLR2K is am ember of the archaeal
RpoP/eukaryotic RPC10 RNA polymerase subunit family. POLR2K is one of the
smallest subunits of RNA polymerase II, the polymerase is responsible for synthesizing
messenger RNA in eukaryotes. In addition, the other two DNA-directed RNA
polymerases share this subunit.
Description
POLR2K Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 81 amino acids (1-58 a.a) and having a molecular mass of 9.4kDa.
POLR2K is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
POLR2K protein solution (0. 5mg/ml) containing Phosphate
buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMDTQKDV QPPKQQPMIY ICGECHTENE IKSRDPIRCR ECGYRIMYKK RTKRLVVFDA R.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
