Catalogue number
PRO-1761
Synonyms
Ring Finger Protein 34, E3 Ubiquitin Protein Ligase, RING
Finger Protein 34, Caspase Regulator CARP1, Caspases-8 And -10-Associated RING
Finger Protein 1, FYVE-RING Finger Protein Momo, Human RING Finger Homologous
To Inhibitor Of Apoptosis Protein, CARP-1, hRFI, RING Finger Protein RIFF, RFI,
CARP1, RIF, RIFF, E3 Ubiquitin-Protein Ligase RNF34, EC 6.3.2.
Introduction
Ring
Finger Protein 34 (RNF34) has E3 ubiquitin-protein ligase activity. RNF34 has a
RINF finger, a motif recognized to be involved in protein-protein and protein-DNA
interactions. RNF34 regulates the levels of CASP8 and CASP10 by targeting them
for proteasomal degradation. In addition, RNF34 protects cells against
apoptosis induced by TNF. RNF34 also binds phosphatidylinositol 5-phosphateand
phosphatidylinositol 3-phosphate. Alternatively splicing results in multiple
transcript variants encoding distinct isoforms.
Description
RNF34 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 396 amino acids (1-373a.a) and having a molecular mass of 44.2kDa.
RNF34 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
RNF34 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPE
FSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLM
RLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAP
SATMSSFQGE LMDGDQTSRS GVPAQVQSEI TSANTEDDDD DDDEDDDDEE ENAEDRNPGL SKERVRASLS
DLSSLDDVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERLQL QDEEDDSLCR ICMDAVIDCV
LLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
