Catalogue number
PRO-1115
Synonyms
Vesicle transport through interaction with t-SNAREs homolog 1B, Vesicle transport v-SNARE protein Vti1-like 1, Vti1-rp1, VTI1B, VTI1, VTI1L, VTI1L1, VTI2, v-SNARE, VTI1-LIKE.
Introduction
VTI1B (v-SNARE) mediates vesicle transport pathways via interactions with t-SNAREs on the target membrane. These interactions are meant to facilitate aspects of the specificity of vesicle trafficking and to stimulate fusion of the lipid bilayers. VTI1B may be involved in increased secretion of cytokines connected with cellular senescence.
Description
VTI1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 231 amino acids (1-208 a.a) and having a molecular mass of 26.3kDa (Molecular weight on SDS-PAGE will appear higher).
VTI1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
VTI1B protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.15M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMASSAAS SEHFEKLHEI FRGLHEDLQG VPERLLGTAG TEEKKKLIRD FDEKQQEANE TLAEMEEELR YAPLSFRNPM MSKLRNYRKD LAKLHREVRS TPLTATPGGR GDMKYGIYAV ENEHMNRLQS QRAMLLQGTE SLNRATQSIE RSHRIATETD QIGSEIIEEL GEQRDQLERT KSRLVNTSEN LSKSRKILRS MSRKVTTNKL L.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
