Catalogue number
PRO-1461
Synonyms
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor
35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary
factor small subunit, U2AF35, U2AFBP, FP793, RNU2AF1, RN.
Introduction
U2AF1
is a member of the splicing factor SR family. U2 auxiliary factor, comprising a
large and a small
subunit, is a non-snRNP protein essential for the binding of U2 snRNP to the
pre-mRNA branch site.U2AF1 is the small subunit which plays a vital role in
both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between
the large subunit and proteins bound to the enhancers. U2 small nuclear RNA
auxiliary factor 1 (U2AF1) is a protein-coding gene. Among the diseases which
are associated with U2AF1:Myoclonus Epilepsy and progressive Myoclonus Epilepsy.
Description
U2AF1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 263 amino acids (1-240 a.a) and having
a molecular mass of 30.3kDa.
U2AF1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
U2AF1 protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL
IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF
RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR
DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
