Catalogue number
PRO-1767
Synonyms
CD47
Molecule, Antigenic Surface Determinant Protein OA3, CD47 Antigen (Rh-Related
Antigen, Integrin-Associated Signal Transducer), Antigen Identified by
Monoclonal, Antibody 1D8, Integrin Associated Protein, Integrin-Associated
Protein, Rh-Related, Antigen, CD47 Glycoprotein, MER6, IAP, Integrin-Associated
Signal Transducer, Leukocyte Surface Antigen CD47, CD47 Antigen, Protein MER6, OA3.
Introduction
CD47, functions in cell adhesions by performing as an adhesion receptor for THBS1 on platelets, furthermore CD47 plays a role in the modulation of integrins. In addition, CD47 takes a vital part in memory formation as well as synaptic plasticity in the hippocampus. Receptor for SIRPA avoids maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 prevents premature elimination of red blood cells, and it is also implicated in membrane permeability changes induced following virus infection.
Description
CD47 Human
Recombinant produced in Sf9 Insect cell is a single, non-glycosylated
polypeptide chain containing 362 amino acids (19-141aa.a) and having a
molecular mass of 40.9kDa (Migrates at 40-57kDa on SDS-PAGE under reducing
conditions).
CD47 is fused to a 239 amino acid hIgG-His-tag at
C-terminus & purified by proprietary chromatographic techniques.
Source
Sf9, Insect cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CD47 protein
solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10%
glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
QLLFNKTKSV
EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL
KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNELEPKSCD KTHTCPPCPA PELLGGPSVF
LFPPKPKDTL MISRTPEVTC VVVDVSHEDP
EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC
KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY
KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
