Catalogue number
CYT-313
Synonyms
gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4.
Introduction
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E- cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.
Description
CD-4 Human Recombinant encoding amino acids 26-226, shows a 48kDa band on SDS-PAGE.
CD4 is fused to a GST tag at N-terminus and purified by standard chromatography techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered liquid.
Formulation
Recombinant Human protein at 100µg/ml in 50mM Tris-HCl, (pH-7.5) and 10mM L-glutathione.
Stability
Store vial at -20oC to -80oC. When stored at the recommended temperature, this protein is stable for 12 months.
Please avoid freeze-thaw cycles.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
