Catalogue number
CYT-932
Synonyms
Tumor Necrosis Factor Receptor Superfamily, Member 11a,
NFKB Activator, Loss Of Heterozygosity, 18, Chromosomal Region 1, Osteoclast
Differentiation Factor Receptor,
Receptor Activator Of
NF-KB, Paget Disease Of Bone 2, ODFR, RANK, Tumor Necrosis Factor Receptor
Superfamily, Member 11a, Activator Of NFKB, Receptor Activator Of Nuclear
Factor-Kappa B, CD265 Antigen, LOH18CR1, TRANCER, CD265, OPTB7, OSTS, PDB2, FEO,
OFE, Tumor necrosis factor receptor superfamily member 11A.
Introduction
RANK, is part of the tumor necrosis factor receptor family. RANK is
widely expressed with uppermost levels in the skeletal muscle, thymus, liver,
colon, small intestine, adrenal gland as well as dendritic cells. Furthermore,
in activated human peripheral blood T lymphocytes, RANK expression is induced
by IL4 and TGF-b.
Description
RANK produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 427 amino acids (28-212a.a.) and
having a molecular mass of 47.6kDa.
(Molecular size on SDS-PAGE will appear at
approximately 40-57kDa).
RANK is expressed with a 242
amino acid hIgG-His-tag at C-Terminus and purified by proprietary
chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RANK protein solution (1mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPLQIAPPC
TSEKHYEHLG RCCNKCEPGK YMSSKCTTTS DSVCLPCGPD EYLDSWNEED KCLLHKVCDT GKALVAVVAG
NSTTPRRCAC TAGYHWSQDC ECCRRNTECA PGLGAQHPLQ LNKDTVCKPC LAGYFSDAFS STDKCRPWTN
CTFLGKRVEH HGTEKSDAVC SSSLPARKPP
NEPHVYLPLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT
PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK
ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ
PENNYKTTPP VLDSDGSFFL
YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPGKHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
