Catalogue number
PRO-2157
Synonyms
Leucine Zipper Transcription Factor-Like 1, BBS17, Leucine
Zipper Transcription Factor-Like Protein 1, Leucine zipper transcription
factor-like protein 1.
Introduction
Leucine
Zipper Transcription Factor-Like 1, also known as LZTFL1 is a ubiquitously
expressed protein which localizes to the cytoplasm. LZTFL1 interacts with Bardet-Biedl
Syndrome proteins (BBS) and during its interaction with BBS protein complexes,
regulates protein trafficking to the ciliary membrane. Nonsense mutations in
LZTFL1 cause a form of Bardet-Biedl Syndrome; a ciliopathy characterized partly
by polydactyly, obesity, cognitive impairment, hypogonadism, and kidney failure. LZTFL1 also acts as a tumor
suppressor; possibly by interacting with E-cadherin as well as the actin cytoskeleton
and in this manner regulating the transition of epithelial cells to mesenchymal
cells.
Description
LZTFL1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 322 amino acids (1-299 a.a) and having a molecular
mass of 37.0kDa.
LZTFL1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
LZTFL1 protein solution (1mg/ml) containing PBS buffer
(pH 7.4), 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAELGLN EHHQNEVINY MRFARSKRGL RLKTVDSCFQ DLKESRLVED
TFTIDEVSEV LNGLQAVVHS EVESELINTA YTNVLLLRQL FAQAEKWYLK LQTDISELEN RELLEQVAEF
EKAEITSSNK KPILDVTKPK LAPLNEGGTA ELLNKEILRL QEENEKLKSR LKTIEIQATN ALDEKSKLEK
ALQDLQLDQG NQKDFIKAQD LSNLENTVAA LKSEFQKTLN DKTENQKSLE ENLATAKHDL LRVQEQLHMA
EKELEKKFQQ TAAYRNMKEI LTKKNDQIKD LRKRLAQYEP ED.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
