Catalogue number
PRO-2120
Synonyms
Coatomer Protein Complex, Subunit Epsilon, Epsilon-Coat
Protein, Epsilon-COP, Coatomer Epsilon Subunit, Coatomer Subunit Epsilon, Epsilon
Coat Protein, Coatomer subunit epsilon.
Introduction
Coatomer Protein Complex Subunit Epsilon, also known as COPE is an epsilon subunit of
coatomer protein complex. Coatomer is a cytosolic protein complex which binds to dilysine motifs
and reversibly links with Golgi
non-clathrin-coated vesicles. COPE is necessary for budding
from Golgi membranes, and is also essential for the retrograde Golgi-to-ER
transport of dilysine-tagged proteins. Coatomer
complex contain at least the alpha, beta, beta', gamma, delta, epsilon and zeta
subunits. Alternatively spliced transcript variants
encoding dissimilar isoforms have been identified for
COPE.
Description
COPE Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 331 amino acids (1-308 a.a) and
having a molecular mass of 36.9kDa.
COPE is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
COPE protein solution (0.25 mg/ml) containing Phosphate
buffered saline (pH7.4),
20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPPAPG PASGGSGEVD ELFDVKNAFY IGSYQQCINE AQRVKLSSPE
RDVERDVFLY RAYLAQRKFG VVLDEIKPSS APELQAVRMF ADYLAHESRR DSIVAELDRE MSRSVDVTNT
TFLLMAASIY LHDQNPDAAL RALHQGDSLE CTAMTVQILL
KLDRLDLARK ELKRMQDLDE DATLTQLATA WVSLATGGEK LQDAYYIFQE
MADKCSPTLL LLNGQAACHM AQGRWEAAEG LLQEALDKDS GYPETLVNLI VLSQHLGKPP EVTNRYLSQL
KDAHRSHPFI KEYQAKENDF DRLVLQYAPS A.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
