Catalogue number
PRO-1060
Synonyms
LSM3 homolog U6 small nuclear RNA associated (S. cerevisiae), U6 snRNA-associated Sm-like protein LSm3, SMX4, USS2, YLR438C.
Introduction
LSM3 is a member of snRNP Sm proteins family which were identified in a large number of organisms based on sequence homology. Sm-like proteins have a Sm sequence motif that holds 2 regions detached by a linker of variable length that fold as a loop. The Sm-like proteins form a stable heteromer found in tri-snRNP particles that are vital for pre-mRNA splicing.
Description
LSM3 Human Recombinant produced in E. coli is a single polypeptide chain containing 125 amino acids (1-102) and having a molecular mass of 14.3kDa.
LSM3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The LSM3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 2mM DTT and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMADDVDQ QQTTNTVEEP LDLIRLSLDE RIYVKMRNDR ELRGRLHAYD QHLNMILGDV EETVTTIEID EETYEEIYKS TKRNIPMLFV RGDGVVLVAP PLRVG
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
