Catalogue number
PRO-2396
Synonyms
Hop, p60, Sti1, STI1, mSTI1, Stress-induced-phosphoprotein
1, Hsc70/Hsp90-organizing protein.
Introduction
STIP1 is an adaptor protein that mediates the functions of HSP70 & HSP90 in protein folding. STIP1 supports the transfer of proteins from HSP70 to HSP90 by binding together HSP90 and substrate-bound HSP70. STIP1 stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones. STIP1 genetic variations are involved in regulating corticosteroid response in asthmatic subjects with reduced lung function.
Description
STIP1 Mouse Recombinant produced in E.coli is a single,
non-glycosylated polypeptide chain containing 563 amino acids (1-543) and
having a molecular mass of 64.7kDa. STIP1 is fused to a 20 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear colorless solution.
Formulation
The STIP1 solution (1mg/ml) contains 20mM Tris-HCl(pH8.0),
0.1M NaCl, 1mM DTT and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH
MEQVNELKEK GNKALSAGNI DDALQCYSEA IKLDPQNHVL YSNRSAAYAK KGDYQKAYED GCKTVDLKPD
WGKGYSRKAA ALEFLNRFEE AKRTYEEGLK HEANNLQLKE GLQNMEARLA ERKFMNPFNL PNLYQKLEND
PRTRSLLSDP TYRELIEQLQ NKPSDLGTKL QDPRVMTTLS VLLGVDLGSM DEEEEAATPP PPPPPKKEPK
PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDKALKHYDR AKELDPTNMT YITNQAAVHF EKGDYNKCRE
LCEKAIEVGR ENREDYRQIA KAYARIGNSY FKEEKYKDAI HFYNKSLAEH RTPDVLKKCQ QAEKILKEQE
RLAYINPDLA LEEKNKGNEC FQKGDYPQAM KHYTEAIKRN PRDAKLYSNR AACYTKLLEF QLALKDCEEC
IQLEPTFIKG YTRKAAALEA MKDYTKAMDV YQKALDLDSS CKEAADGYQR CMMAQYNRHD SPEDVKRRAM
ADPEVQQIMS DPAMRLILEQ MQKDPQALSE HLKNPVIAQK IQKLMDVGLI AIR.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
