Catalogue number
PRO-459
Synonyms
JIP, DPC4, MADH4, SMAD-4, DPC-4, MADH-4, Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, SMAD 4, hSMAD4, Deletion target in pancreatic carcinoma 4, SMAD4, SMAD family member 4.
Introduction
SMAD4 is part of the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD4 promotes the binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides the function of activation required for SMAD1 or SMAD2 to stimulate transcription acts as a tumor suppressor. SMAD4 is a target molecule for functional inactivation in cervical cancer. SMAD4 is an important biomarker for malignant transformation atakes part in inducing apoptosis by modulating Bcl-2/Bax balance.
Description
SMAD4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 572 amino acids (1-552) and having a molecular mass of 62.6 kDa. SMAD4 is fused to a 20 amino acid His-Tag at N-Terminus and purified by standard chromatography techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The SMAD4 protein solution contains 20mM Tris-HCl pH-8, and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LD.
Purity
Greater than 90.0% as determined by SDS-PAGE.
References
Title:Transforming Growth Factor β-Induced Reactivation of Epstein-Barr Virus Involves Multiple Smad-Binding Elements Cooperatively Activating Expression of the Latent-Lytic Switch BZLF1 Gene .
Publication:Published ahead of print 18 May 2011, doi: 10.1128/JVI.01197-10 J. Virol. August 2011 vol. 85 no. 15 7836-7848
Link:http://jvi.asm.org/content/85/15/7836.full
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
