Catalogue number
CYT-914
Synonyms
Angiopoietin-related protein 7, Angiopoietin-like protein 7.
Introduction
Angiopoietin-related protein 7 (ANGPTL7), belongs to the angiopoietin-like family of molecules. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. ANGPTL7 production is up-regulated in trabecular meshwork cells by glucocorticoids and TGF-Beta and in cartilage by TNF-alpha.
Description
ANGPTL7 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 324 amino acids (22-337 a.a.) and
having a molecular mass of 37.5kDa (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
ANGPTL7 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ANGPTL7 protein solution (0.25mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4
weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
QKPHKRKTQL KAAGCCEEMR ELKAQVANLS SLLGELSRKQ ESDWVSVVMQ
VMELESSSKH MESRLSTAES KYSEMNNQID IMQLQAAQTV TQTSADAIYD CSSLYQKNYR ISGVYKLPPD
EFLGSPELEV FCDMETSGGG WTIIQRRKSG LVSFYQDWRQ YKQGFGSIRG DFWLGNEHIH RLTRQPSRLR
VELEDWEGNA RYAEYSYFAL GNELNSYRLF LGNYSGNVGK DALLYHNNTV FSTKDKDNDN CLDKCAQLRK
GGYWYNCCTD SNLNGVYYRL GEHRKHMDGI SWYGWHGANY SLKRVEMKIR PEAFKPLEHH HHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
