Catalogue number
PRO-1317
Synonyms
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, T3E, TCRE.
Introduction
CD3-epsilonpolypeptide (CD3E) along with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, creates the T-cell receptor-CD3 complex. This complex has an imperative role in coupling antigen recognition to a number of intracellular signal-transduction pathways. The genes encoding the epsilon, gamma delta polypeptides are found in the same cluster on chromosome 11. The epsilon polypeptide plays a vital role in T-cell development. CD3E gene defects cause immunodeficiency. CD3E is also linked to a susceptibility to type I diabetes in women.
Description
CD3e Human Recombinant produced in E. coli is a single polypeptide chain containing 127 amino acids (23-126) and having a molecular mass of 14.1kDa.
CD3e is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The CD3e solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSDGNEEMG GITQTPYKVS ISGTTVILTC PQYPGSEILW QHNDKNIGGD EDDKNIGSDE DHLSLKEFSE LEQSGYYVCY PRGSKPEDAN FYLYLRARVC ENCMEMD.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
