Catalogue number
PRO-1718
Synonyms
Nucleic Acid Binding Protein 1, OBFC2A, Sensor Of
Single-Strand DNA Complex Subunit B2, Single-Stranded DNA-Binding Protein 2, SSB2,
Oligonucleotide/Oligosaccharide-Binding Fold Containing 2A, Nucleic
Acid-Binding Protein 1, Oligonucleotide/Oligosaccharide-Binding Fold-Containing
Protein 2A, Sensor Of SsDNA Subunit B2, SOSS-B2, SOSS Complex Subunit B2, hSSB2.
Introduction
Nucleic acid binding protein 1 is a part of the SOSS complex, a multiprotein complex
which functions downstream of the MRN complex to promote DNA repair and G2/M
checkpoint. In the SOSS complex,
NABP1 acts as a sensor of single-stranded DNA which binds to single-stranded
DNA, in particular
to polypyrimidines. The SOSS complex links with DNA lesions and affects diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint
activation, recombinational repair and maintenance of genomic stability. NABP1 is essential for
efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and
ATM-dependent signaling pathways.
Description
NABP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (1-204 a.a) and having a molecular mass of 24.8kDa.
NABP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
NABP1 protein solution (0.5mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0) 0.2M, NaCl, 50% glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMNRVNDP LIFIRDIKPG LKNLNVVFIV LEIGRVTKTK DGHEVRSCKV
ADKTGSITIS VWDEIGGLIQ PGDIIRLTRG YASMWKGCLT LYTGRGGELQ KIGEFCMVYS EVPNFSEPNP
DYRGQQNKGA QSEQKNNSMN SNMGTGTFGP VGNGVHTGPE SREHQFSHAG RSNGRGLINP QLQGTASNQT VMTTISNGRD PRRAFKR.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
