Catalogue number
PRO-2256
Synonyms
DLK1, Delta-Like 1 Homolog (Drosophila), PREF1, DLK-1, FA1, DLK, PG2, Delta-Like Homolog (Drosophila), Protein Delta Homolog 1, Preadipocyte Factor 1, Fetal Antigen 1, Secredeltin, DELTA1, Pref-1, ZOG.
Introduction
Delta-Like 1, also known as DLK1 is a
member of the Notch/Delta/Serrate family of epidermal growth factor (EGF)-like
repeat-containing proteins which act as ligands in the Notch signaling pathway.
Furthermore, DLK1 has six highly conserved epidermal-growth-factor motifs and
it also contains up to ten O-glycosylation and N-glycosylation locations, six
of them are differentially glycosylated.
Description
DLK1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 288 amino acids (24-303 a.a) and having a molecular mass of 30.7kDa (Migrates at 28-40kDa on SDS-PAGE under reducing conditions).
DLK1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered clear solution.
Formulation
DLK1 protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
AECFPACNPQ NGFCEDDNVC RCQPGWQGPL CDQCVTSPGC LHGLCGEPGQ CICTDGWDGE LCDRDVRACS SAPCANNGTC VSLDDGLYEC SCAPGYSGKD CQKKDGPCVI NGSPCQHGGT CVDDEGRASH ASCLCPPGFS GNFCEIVANS CTPNPCENDG VCTDIGGDFR CRCPAGFIDK TCSRPVTNCA SSPCQNGGTC LQHTQVSYEC LCKPEFTGLT CVKKRALSPQ QVTRLPSGYG LAYRLTPGVH ELPVQQPEHR ILKVSMKELN KKTPLLTEGQ LEHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
