Catalogue number
PRO-873
Synonyms
RAB14 member RAS oncogene family, FBP, bA165P4.3 (member RAS oncogene family),
F protein-binding protein 1, ras-related protein Rab-14, small GTP binding protein RAB14.
Introduction
RAB14 belongs to the RAB protein family, low molecular mass monomeric GTPases which is found on the cytoplasmic surfaces of distinct membrane bound organelles. RAB14 has a role in vesicular trafficking and neurotransmitter release throughout the body and is expressed at high levels in brain, lung, kidney, spleen and thymus. In addition, RAB14 takes part in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.
Description
RAB14 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-215) and having a molecular mass of 26.3 kDa.
The RAB14 is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
RAB14 is supplied as a sterile filtered clear solution.
Formulation
RAB14 protein 0.5mg/ml is supplied in 20mM Tris-HCL, pH-8, 0.1M NaCl, 1mM DTT and 20% Glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMATAPYN YSYIFKYIII GDMGVGKSCL LHQFTEKKFM ADCPHTIGVE FGTRIIEVSG QKIKLQIWDT AGQERFRAVT RSYYRGAAGA LMVYDITRRS TYNHLSSWLT DARNLTNPNT VIILIGNKAD LEAQRDVTYE EAKQFAEENG LLFLEASAKT GENVEDAFLE AAKKIYQNIQ DGSLDLNAAE SGVQHKPSAP QGGRLTSEPQ PQREGCGC
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
