Catalogue number
PRO-346
Synonyms
Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817, Troponin C slow skeletal and cardiac muscles, TN-C, TNNC1, TNNC, TNC, CMD1Z.
Introduction
Troponin Complex is a heteromeric protein playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, Troponin I, Troponin T and TroponinC. Each subunit is responsible for part of Troponin Complex function. E.g. Troponin I inhibits ATP-ase activity of acto-myosin.Troponin T and Troponin Iare presented in cardiac muscles in different forms than in skeletal muscles. Purified subunits of rcTnI, rcTnC and rcTnT are recomplexed in vitro under appropriate conditions.
Description
Recombinant Single Chain Cardiac Troponin I-C 2nd generation produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa28-110-Linker-cTnC), having a molecular weight of 30kDa.
1mg of scIC2 is equivalent to 0.82mg of full length human cardiac TNI on a molar basis.
The scIC2 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colourless liquid formualtion.
Formulation
The protein solution contains 20mM Tris-HCl, 250mM NaCl and 50% glycerol, pH 7.5.
Stability
scIC2 although stable at 10°C for 7 days, should be stored below -18°C.
Please prevent freeze-thaw cycles.
Purity
Greater than 91.0% as determined by SDS-PAGE.
Applications
Immunoassays and western blot.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
