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Biotin Anti-Human IgA1 Rabbit Monoclonal Antibody, Clone RM124
品牌:RevMAb
货号:31-1026-02
规格:50 ug
货期:电询

商品详情 参考文献 相关资料

Product Details
Product Name Biotin Anti-Human IgA1 Rabbit Monoclonal Antibody [RM124]
Product Description Biotinylated Rabbit monoclonal to Human IgA1
Catalog No. 31-1026-02
Clone Name RM124
Product Data Sheet Biotin Anti-Human IgA1 Rabbit Monoclonal Antibody Data Sheet RM124
Specificity RM124 reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Immunogen Human IgA
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.1ug/mL-1ug/mL.
Keywords
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Product Images
Immunohistochemistry

Immunohistochemistry of Human Tonsil Tissue using
Anti-Human IgA1 antibody RM124.

Sandwich

Sandwich ELISA using RM124 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

Sandwich

Sandwich ELISA using RM124 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.

ELISA

ELISA of human immunoglobulins shows RM124
reacts only to Human IgA. Very slightly cross
reacts with IgA2. No cross reactivity with Human
IgG, IgM, IgD, or IgE. The plate was coated with
50 ng/well of different immunoglobulins. 200 ng/mL,
50 ng/mL, or 10 ng/mL of RM124 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.

A

A titer ELISA using RM124. The plate was coated
with different amounts of human IgA1. A serial dilution
of RM124 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.


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