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Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151
品牌:RevMAb
货号:31-1059-00
规格:100 ug
货期:电询

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Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM151]
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9)
Catalog No. 31-1059-00
Clone Name RM151
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM151
Specificity RM151 reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9).
Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt.
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.25 ug/mL – 1 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images
RM151

RM151 specifically reacts to Histone H3 dimethylated
at Lysine 9 (K9me2). No cross reactivity with non
-modified Lysine 9 (H3 1-19), monomethylated
Lysine 9 (K9me1), trimethylated Lysine 9
(K9me3), or other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM151
at 0.5 ug/mL, showed a band of histone H3
dimethylated at Lysine 9 (K9me2) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K9me2
antibody (RM151, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Dimethyl-Histone H3
(Lys9) Rabbit mAb RM151 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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