Immunohistochemistry of mouse liver and kidney using 0.5ug/mL of RevMAb anti-PEG Clone RM105 biotin conjugate, followed by an HRP conjugated streptavidin. The mouse was injected with PEG-BSA or BSA 3 hours before sampling.
ELISA of PEGylated BSA using Biotinylated anti-PEG rabbit monoclonal antibody clone RM105. The ELISA plate was coated with 50 uL/well of BSA-PEG at 1 ug/mL, 0.33 ug/mL, 0.11 ug/mL, and 0.04 ug/mL. Different concentrations of Biotinylated anti-PEG mAb clone RM105 was used as the primary antibody, followed by an AP conjugated Streptavidin.
Western blot of BSA and PEGylated BSA (mPEG 5 kDa) using 0.1 ug/mL of anti-PEG RevMAb clone RM105.
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