RM186 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono- methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody (clone RM186) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
BIOLEAF热搜 BIOLEAF试剂盒 BIOLEAF ELISA BIOLEAF试剂 BIOLEAF品牌 BIOLEAF抗体 BIOLEAF耗材 BIOLEAF小仪器
sitemap 细胞库查询 危险品图标 本公司网站所展示销售的产品仅供科研!
沪公网安备 31011202007338号