Detection of human IgG1 in monkey serum, using RM117 (capture) and biotin-RM129 (detection) as a Sandwich ELISA pair. HRP conjugated streptavidin and TMB were used to yield the colorimetric reaction.
ELISA showing RM129 reacts only to kappa and lambda light chain of all human immunoglobulins, not to mouse IgG, rat IgG, or goat IgG.
ELISA showing RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
A titer Sandwich ELISA using Biotinylated RM129 as the detection antibody. The plate, coated with the capture antibody anti-human IgG RM116, was loaded with different amounts of human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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