Product Details
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Product Name
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Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody [RM116]
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Product Description
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Rabbit Monoclonal to Human Gamma Heavy Chain
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Catalog No.
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31-1018-00
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Clone Name
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RM116
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Product Data Sheet
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Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody Data Sheet RM116
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Specificity
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RM116 reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
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Immunogen
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Human IgG
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Application
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Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Human
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Conjugate
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None
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Intended Use
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Research Use Only
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.
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
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Keywords
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.
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Product Images
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Immunohistochemistry of Human Tonsil using
Anti-Gamma Heavy Chain antibody RM116.
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ELISA of human immunoglobulins shows RM116
reacted to the γ1, γ2, γ3, γ4 heavy chain of human
IgGs, and the Fc of human IgG. No cross reactivity with
other human heavy chains, mouse IgG, rat IgG,
or goat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL or 50 ng/mL
of RM116 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as the
secondary antibody.
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A titer ELISA using RM116. The plate was coated
with different amounts of human IgG1. A serial dilution
of RM116 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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Sandwich ELISA using RM116 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1ug/mL),
followed by an alkaline phosphatase conjugated streptavidin.
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Sandwich ELISA using RM116 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1ug/mL),
followed by an alkaline phosphatase conjugated streptavidin.
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Cross-activity data of RevMAb anti-human IgG antibodies against
monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL,
50uL/well) of Rhesus monkey IgG (protein A purified from serum).
A serial dilution of anti-human IgG (RM116), anti-human IgG1
(RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119),
(anti-human IgG4 (RM120), anti-human kappa (RM126), and
anti-human lambda (RM127) was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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