Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag 31-1048-00|产品详情|进口试剂采购网

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Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag

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Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag

品牌：RevMAb

货号：31-1048-00

规格：100 ug

货期：电询

应用：

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Product Details
Product Name	Anti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody [RM146]
Product Description	Rabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No.	31-1048-00
Clone Name	RM146
Product Data Sheet	Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146
Specificity	RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogen	Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application	Western Blot Immunoprecipitation Immunocytochemistry Flow Cytometry Immunohistochemistry
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.1 ug/mL – 1 ug/mL; Immunoprecipitation: 0.5 ug/mL – 2 ug/mL; Immunocytochemistry 0.5 ug/mL – 2 ug/mL; Flow Cytometry: 0.5 ug/mL – 2 ug/mL; Immunohistochemistry; 0.1 ug/mL-1 ug/mL.
Keywords
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Product Images
	Western blot of 293T cells: (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y Using RevMAb Clone RM146 at 0.2 ug/mL.
	Immunoprecipitation of 293T cells expressing a His-Tag fusion protein by RevMAb clone RM146, was blotted with RM146. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM146.
	Immunohistochemistry staining of naïve HepG2 cells(Negative control) using anti-His-Tag antibody, RM146.
	Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, RM146.