Immunohistochemical staining of formalin fixed and paraffin embedded human Glioblastoma Multiforme tissue sections with or without EGFRvIIII using anti-EGFRvIII antibody (RM419) at 1:100 dilution.
Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates, using anti-EGFRvIII antibody (RM419) and anti-EGFRct (RM294) at 1:1000 dilution.
Flow cytometry analysis of DKMG and 293 cells, using anti-EGFRvIII antibody (RM419) at 1:50 dilution.
ELISA analysis showing RM419 does not react to wild type EGFR protein. The plate was coated with recombinant EGFR variant III and EGFRwt proteins. A serial dilution of anti-EGFRvlll (RM419) or anti-EGFR (RM229) antibodies were used.
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