Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/ Okadaic Acid, using RevMAb Clone RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).
Western blot of serum-starved A431 cells non- treated or treated with Calyculin A/Okadaic Acid, using RevMAb Clone RM102 at 1/2000 dilution.
Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by RevMAb clone RM102 at 1/500 dilution, and then blotted with RM102. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102.
RevMAb rabbit monoclonal antibody clone RM102 recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross- reactivity with phosphorylated serine.
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