RM235 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non- modified Lysine 20 or other acetylated Lysines in histone H2B.
Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using RM235 at 0.5 ug/mL.
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody (clone RM235) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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