Total Collagen Assay Now collagen can be easily determined by any biochemical laboratory The Total Collagen assay kit is based on the quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. Usually for this analysis upon hydrolysis removal of HCl is needed, which is a time-consuming step and/or needs special equipment. The QuickZyme Total Collagen assay is the only hydroxyproline-based assay which does not need this laborious step, resulting in a fast (<2 hrs) and easy (96-well plate format) assay following the hydrolysis step. Total Collagen Assay specifications Quantitative measurement of all types of collagen, species independent. Samples: tissue culture supernatants, cellular extracts, tissue homogenates, tissues. Colorimetric measurement of hydroxyproline content Range: 6 to 300 μg/ml. Sensitivity: 2.5 µg/ml Uses rat tail collagen as standard Ease-of-use: Equivalent to ELISA Store at room temperature规格：One 96 well plate kit