Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size.The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals .mTOR is a large protein kinase with two different complexes.One complex contains mTOR, G beta L and raptor, which is a target of rapamycin.The other complex, insensitive to rapamycin, includes mTOR, G beta L, Sin1 and rictor .The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro .This phosphorylation is essential for full Akt/PKB activation.Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes .This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR .

Target	RICTOR
Reactivity	Human, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Tested Applications	ELISA, WB
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Purified recombinant fragment of human RICTOR expressed in E. coli.
Isotype	IgG1
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	192 kDa
Buffer	Ascitic fluid containing 0.03% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.