Smad4 antibody detects endogenous levels of total Smad4. Smad4 transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Target	Smad4
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB
Recommended dilutions	WB: 1/500 - 1/3000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	A synthesized peptide derived from human Smad4.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	60 kDa
Swiss Prot	Q13485
Buffer	Rabbit IgG in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.