Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Target	RAF1 (pS339)
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Tested Applications	DB
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Human RAF1 (phospho-Ser339).
Purification	Peptide Affinity Purified Rabbit Polyclonal Antibody.
Isotype	IgG
Conjugation	Unconjugated
Specificity	This RAF1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S339 of human RAF1.
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Swiss Prot	P04049
Buffer	PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.