CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis.Cdc25, when phosphorylated on serine 216 by CHK1 becomes bound by an adaptor protein in the cytoplasm.Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1.Consequently, a cell is prevented from entering mitosis.CHK1 can also phosphorylate p53 at Ser20 in vitro.

Target	CHK1
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Tested Applications	ELISA, WB
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Purified recombinant fragment of CHK1 expressed in E. coli.
Purification	Purified antibody
Isotype	IgG1
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	56 kDa
Buffer	PBS containing 0.03% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.