Phospho-AurB (Thr232) Antibody detects endogenous levels of AurB only when phosphorylated at Threonine 232. Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation.

Target	AurB (pT232)
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Tested Applications	WB, IHC, IF/ICC
Recommended dilutions	WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	A synthesized peptide derived from human AurB around the phosphorylation site of Threonine 232.
Purification	Immunogen affinity purified.
Conjugation	Unconjugated
Storage	Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight	39 kDa
Swiss Prot	Q96GD4
Enzyme Commission Number	EC 2.7.11.1
Buffer	Rabbit IgG in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.