The monoclonal antibody is directed against the CD22- antigen, which is expressed on human B lymphocytes. Normal T-cells, polymorph nuclear cells, monocytes and platelets were found to be negative. Cell lines SB, Raji and NALM-1 were found to be positive. Most CALLA-ALL were found to be positive. The reaction pattern to CLL and NHL is variable from weak to negative. PLL is clearly positive and Hairy Cell Leukaemia is very strong positive with this antibody. Reacts with the 130-140 kD glycosylated type I integral membrane protein present on the surface of mature B cells. CD22 is expressed in the cytoplasm of virtually all B cells except plasma cells. CD45RO antigen on T cells and CD75 antigen on B cells have been identified as ligands for CD22. CD22 has been reported to participate in B-cell activation and also as an adhesion molecule. Although the immunobiology of this antigen has not been fully elucidated, reports indicate that ligation of CD22 induces constitutive internalization of the molecule followed by complete degradation.

Target	CD22
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Tested Applications	FCM
Recommended dilutions	FCM: 20 μl/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Immunogen	Hairy Cell Leukemia cells.
Purification	Affinity Chromatography
Form	Liquid
Isotype	IgG1
Conjugation	PE
Specificity	CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.
Storage	Store in the dark at 2-8 °C.
Molecular Weight	130 - 140 kDa
Swiss Prot	P20273
GeneID	933
Buffer	The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide
Availability	Shipped within 3-5 working days.
Note	This product is for research use only.