GDF-3 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-9.  Unlike most TGF-β family members, GDF-3 and GDF-9 are not disulfide-linked dimers.  GDF-3 is expressed in ***** bone marrow, spleen, thymus, and adipose tissue.  The expression of GDF-3 is upregulated in high-fat-fed wild-type FABP4/aP2 null mice and was associated with obesity, but not with the related hyperglycemia/hyperinsulinemia that characterizes Type 2 diabetes.  Recombinant Human GDF-3 is a 26.0 kDa non-disulfide-linked homodimer containing two 114 amino acid polypeptide chains.

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Growth/Differentiation Factor-3, Vgr-2, UNQ2222/PRO248

AA Sequence (monomer): AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells.  The ED₅₀ for this effect is 100-150 ng/ml.