Interleukin-35 is a glycosylated, heterodimeric protein consisting of the p35 subunit from IL-12 (IL-12α) and the β subunit from IL-27 (EBI3). IL-35 can be expressed by regulatory T-cells (Tregs), macrophages, and certain trophoblast and dendritic cells. It is induced in response to inflammation, and generally acts as an inflammation suppressor. IL-35 suppresses inflammation by exerting multiple activities, including the induction of regulatory T-cells and the suppression of Th17 cells. Recombinant Human IL-35 produced from HEK293 cells is a glycosylated protein, primarily heterodimeric, that migrates as a diffuse band centered at an apparent molecular weight of about 60 kDa by SDS-PAGE analysis under non-reducing conditions. Recombinant Human IL-35 expressed in HEK293 cells contains 406 amino acid residues and has a calculated molecular weight of 45.8 kDa.

Source: HEK293 cells

Synonyms: Interleukin-35

AA Sequence:

α Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS

β Subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

Purity: ≥ 90% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Measured by its ability to inhibit proliferative activity of PBMCs.

Note:

50μg is the largest vial size for this product.
Larger sizes supplied as multiple 50μg vials.