Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholino antisense oligos have shown specific activity against Dengue virus.
Dengue-gold is an antigen used for dengue antibody rapid test. This antigen is genetically developed from 4 dengue subtypes. This antigen can be recognized by most of the dengue antibodies from infected patients.
Dengue Antigen for Rapid Test has over 90% sensitivity and specificity over 90% to detect both dengue IgM and IgG.
Assay Conditions
1. Prepare 528 to 533nm colloid gold, conjugation within 24 hours after colloid gold preparation;
2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 20ug / ml gold and 7.2;
3. Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20oC for 20 min;
4. Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2;
5. Add the supplied blocking reagent to the final concentration to 0.4%;
6. Blocking for 1 hours , then add sucrose to the final concentration to 20%;
7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment);
8. After drying, assemble gold pad and absorbent pad over backing card;
9. Add 10µl serum/plasma and add the supplied running buffer for assay.
10. It is suggested to use our monoclonal anti-human IgG and IgM cat# ant-529 and ant-530 for rapid test development kit.
