UFD1L forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.
UFD1L Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 330 amino acids (1-307) and having a molecular mass of 36.9kDa.
UFD1L is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
The UFD1L solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 30% glycerol.