Greater than 95% as determined by SDS-PAGE. UBE2D2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-147) and having a molecular mass of 18.9 kDa. The UBE2D2 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. The UBE2D2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.
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