NRAS antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000. Mouse IgG2a heavy chain and;k light chain. Anti-human NRAS mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NRAS amino acids 1-186 purified from E. coli. NRAS antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Mouse Anti Human Monoclonal.