In stock
Convenient, cost effective sampler pack containing four DPc10 peptide derivatives (200µg each) as follows:
DPc10 peptide is a 36-residue peptide corresponding to the Gly2460-Pro2495 region of rabbit RyR2. It interacts with RyR2 and provokes abnormal channel activity usually seen in mutant RYR2 channels associated with CPVT. This peptide can be used to provoke pathological RyR2 behaviour in wild-type RyR2 channels. The peptide binds at an interface between domains of RYR2, “unzipping” these domains and altering channel function. The DPc10 is preserved in RyR2 from all mammalian species. DPc10 (0.5-5 µM) elevates Ca2+-leak, increases Ca2+-spark activity, is pro-arrhythmic, and provokes hypertrophy in cardiac myocytes.
DPc10 inactive peptide is a 36-residue peptide that differs from DPc10 (Gly2460-Pro2495, rabbit) at one position (R2475S) and represents the sequence of a known CPVT mutant RyR2 domain. This peptide binds weakly or fails to bind to the DPc10 interface and does NOT affect channel function. It serves as a negative control for research projects using DPc10 peptide.
DPc10 peptides in the current form are not cell permeable. The products are useful in applications that permit access to RyR2 directly (via whole cell patch, skinned cells, black lipid membrane, vesicles, or upon delivery to cytoplasm).
Read DPc10 Publications
DMSO
Store as supplied at -20°C for up to 1 year. Store desiccated. Make fresh solutions prior to use.
The products were purified in acetonitrile and water containing 0.1% trifluoroacetic acid (TFA) prior to lyophilisation. The products will therefore be present as its trifluoroacetic acid salts.
*Peptides are supplied by net peptide weight. Whilst the purity of the material has been shown to be >95%, the net peptide content is lower due to counterions and residual water present in the material. To compensate for this the amount of peptide weighed out is adjusted so that the amount of active material received is exactly as stated on the vial. This means that the total mass will be in excess of 200µg, but there will be exactly 200µg of the active peptide in the vial. The remainder of the weight is due to the counter ions and residual water.
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