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Yamanaka Factor Set Adenovirus (Human)

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Yamanaka Factor Set Adenovirus (Human)

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	Print Version
Vector	pAdeno
Promoter	CMV
Insert	Yamanaka Factor Set
Picture
Organism	Human
Accession #	Refer to individual adenovirus.
Alias	Oct4, Sox2, KLF4, Myc and GFP control virus.
Protein Type	Wild Type
Functions	Co-expression of Oct4, Sox2, KLF4 and Myc (Yamanaka set) in any somatic cell will lead to iPSC reprogramming. The Yamanaka set of transcription factors provides purified, high titer adenoviruses that offer the highest gene transduction efficiency for iPSC induction. The adenoviral vector will not integrated into host cell genome, avoiding possible mutations associated with vector insertion.
Gene Type	cDNA
Purification	By Ion-Exchange column.
Quality Control	By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming.
Titer	>1x10⁶pfu/mL
Storage	10mM Tris-HCl, pH 8.0. 2.5% glycerol
Shipping	High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induction of pluripotent stem cells from *** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. Induction of pluripotent stem cells from mouse embryonic and *** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al. Nature. 2009 Apr 9;458(7239):771-5. Epub 2009 Mar 1.