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mOCT4 Lysate

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mOCT4 Lysate

商品详情参考文献相关资料

	Print Version
Organism	Mouse
Accession #	P20263
Alias	POU5F1, OCT3, OTF3
Protein Type	Wild Type
Protein Sequence	MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGIGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQARKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSSIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVPVTALGSPMHSN
Protein Family	Belongs to the POU transcription factor family. Class-5 subfamily.
Functions	Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Oct4 is one of the four factors in both the Thomson and the Yamanaka protocol for iPSC reprogramming. The recombinant protein within the lysate has a 6X His tag at N-terminus for purification and 9X Arginine at C-terminus for cell membrane penetration.
Cell Types	Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo.
Protein Location	Nucleus.
Tag	6X His tag at N-terminus and 9X Poly-Arginine at C-terminus
Form	Liquid
Expression System	E.coli
Type	Lysate
Purification	None
Quality Control	By Western Blot Analysis
Concentration	1mg/ml
Storage	DMEM with 2.5% glycerol
Shipping	On dry ice.
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26. Induction of pluripotent stem cells from *** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. Induction of pluripotent stem cells from mouse embryonic and *** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.