OneScript® Plus Reverse Transcriptase (PCR) G177|产品详情|进口试剂采购网

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OneScript® Plus Reverse Transcriptase (PCR)

品牌：Abmgood

货号：G177

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OneScript® Plus Reverse Transcriptase (PCR)

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Description	Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with abm’s OneScript^® Plus Reverse Transcriptase. Engineered to perform under high temperatures (45°C - 55°C), abm’s OneScript^® Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, OneScript^® Plus Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing.
Literature
Application	• Synthesizing cDNA from a ssRNA • DNA primer extension • Sequencing dsDNA • Constructing cDNA library • Constructing libraries for serial analysis for gene expression (SAGE) • Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE) • Producing template for use in RT-PCR or real-time RT-PCR • Labelling 3'-end of duplex DNA via end-filling reactions • Generating probes for hybridization
Form	Enzyme supplied with 5X RT buffer
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Protocol
Concentration	200 U/ul
Storage	Store all components at -20°C.
Notes	This product is ISO-13485 certified.