Poly(A) Polymerase, Yeast E017|产品详情|进口试剂采购网

您好，欢迎来到拜力生物网站！请登录注册

我的订单 | 联系我们 | 在线客服QQ：3012681483 | 客服电话：400-968-7988 | 工作时间：08:30-17:30

主营产品： ELISA试剂盒、抗体、补体血清、兔补体、干扰素、小鼠测温仪、进口试剂、Quidel代理、Swant代理、PBL Assay Science代理

所在位置：首页 > 产品中心 > abmgood > abmgood产品

订购信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Poly(A) Polymerase, Yeast

品牌：Abmgood

货号：E017

规格：

货期：

立即购买加入购物车

Poly(A) Polymerase, Yeast

商品详情参考文献相关资料

	Print Version
Description	Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
Components	Enzyme supplied with 5X Reaction Buffer.
Application	• Labelling of RNA with ATP or cordycepin • Poly(A) tailing of RNA for cloning or affinity purification • Increasing translation of RNA transferred into eukaryotic cells
Image
Concentration	1 U/μl
Storage	Store all components at -20°C.
Storage Buffer	20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Notes	One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C. This product is distributed for laboratory research only. Caution: Not for diagnostic use .