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Anti-Oct4Rabbit Polyclonal Antibody

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Anti-Oct4Rabbit Polyclonal Antibody

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Overview	Print Version
Product Name	Anti-Oct4Rabbit Polyclonal Antibody
Description	Expression of the Oct-4 transcription factor is associated with an undifferentiated phenotype of human and mouse embryonic stem cells and germ cells. Oct-4 (Octamer-4) is a homeodomain transcription factor of the POU family (POU5F1) involved in regulation of pluripotency during normal development and is detectable in embryonic stem (ES) and germ cells. Its expression is associated with an undifferentiated phenotype and tumors. In fact, gene knockdown of Oct4 promotes differentiation, thereby demonstrating a role for this factor in human ES cell self-renewal. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. Oct4 is one of the four key transcription factors (Oct4, Sox2, c-Myc, and KLF4) used to reprogram mouse and human fibroblasts to a pluripotent state as well as generate patient-specific iPS cells. Affinity purified rabbit anti-Oct4 can be used to demonstrate pluripotency of ES and iPS cells.
Subtype	polyclonal
Application	WB , IHC , ELISA
Raised In	Rabbit
Specificity	Observed molecular weight of 50kDa. Positive WB controls detected in HESC cell and 293 cell lysate. Positive IHC control detected in colon cancer cells.
Immunogen	Fusion recombinant protein to POU5F1 (alternative symbols: POU5F1;MGC22487;OCT3;OCT4;OTF3;OTF4). C-term-164aa with GST tag, isolated from E. coli.
Guarantee	abm guarantees that all our Anti-Oct4Rabbit Polyclonal Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Properties
Formulation	Liquid
Quantity	100 μl
Clone	Polyclonal
Storage	This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer	PBS, pH 7.4 with 0.02% sodium azide and BSA
Concentration	100µg/100ul
Purity	Affinity purified
Isotype	IgG
Species Reactivity	human,mouse
Anti-Oct4Rabbit Polyclonal Antibody Images




Specific References
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