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E. coli DNA Polymerase I

品牌：Abmgood

货号：G469

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E. coli DNA Polymerase I

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	Print Version
Description	E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity.
Application	Generation of labeled DNA probes by nick translation Second-strand synthesis of cDNA Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Form	Enzyme supplied with 10X Reaction Buffer
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Concentration	5 U/μl
Storage	Store all components at -20°C.
Storage Buffer	25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
Notes	One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.