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Print Version |
| Alternative Names |
IL-6, IL-6/26 kDa protein, B cell stimulatory factor 2, BSF-2, B cell differentiation factor, BCDF, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HPGF, HSF, MGI-2
Recombinant Human Interleukin-6 (IL6) |
| Description |
IL-6 is a pleiotropic cytokine that participates in a wide variety of biological functions such as acute phase response, inflammation, hematopoiesis, bone metabolism and cancer. While produced mainly by stimulated monocytes, fibroblasts and endothelial cells, IL-6 is also known to be produced by macrophages, T cells, B lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells and keratinocytes, upon stimulation. Unlike murine IL-6 which is inactive on human cells, both human and murine IL-6 are equally active on murine cells. The IL-6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids (designated CD126). Recombinant human IL-6 is a 21 kDa, glycosylated protein that contains two disulfide bridges. |
| Gene Symbol |
IL6 |
| Gene ID |
3569 |
| Accession No. |
P05231 |
| Source |
CHO cells |
| Appearance |
Lyophilized Powder |
| Molecular Weight |
21.0 kDa |
| Endotoxin Level |
<1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Purity |
>95% as determined by SDS-PAGE |
| Bioactivity |
The ED(50) was determined by the dose-dependent stimulation of the proliferation of murine 7TD1 cells was found to be less than 0.1 ng/mL, corresponding to specific activity of 1.0 x 10^7 IU/mg. |
| Formulation |
Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution pH 7.0. |
| Reconstitution |
A quick spin of the vial followed by reconstitution in sterile PBS to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage |
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage |
For research use only. Not for diagnostic or therapeutic use. |
